Four groups of tyrosine hydroxylase-immunoreactive neurons in the ventrolateral medulla of rats, guinea-pigs and cats identified on the basis of chemistry, topography and morphology

Abstract

The data in the preceding paper [Halliday G. M. and McLachlan E.M. (1991) Neuroscience 43, 531–550] suggest that some neurons in the rostral ventrolateral medulla contain some catecholaminesynthesizing enzymes but may not produce catecholamines. The present study addresses this question directly by comparing the anatomical location and morphology of these neurons with those revealed by formaldehyde-induced fluorescence. Catecholamine-containing somata of rats and guinea-pigs have been demonstrated following FAGLU-perfusion in normal untreated animals, in animals pretreated with pargyline (a monoamine oxidase inhibitor), and in animals pretreated with colchicine (to block axoplasmic transport). The number and location of fluorescent somata in the ventrolateral medulla have been determined in serial coronal sections of tissue from the cervical spinal cord to the level of the facial nucleus. Catecholamine-fluorescent neurons at different levels of the ventrolateral medulla varied in their topography and sensitivity to pharmacological manipulation. However, the rostrocaudal distributions in rats and guinea-pigs were quantitatively remarkably similar implying that homologous groups of catecholamine-containing neurons exist. Comparison between these distributions and those of somata stained immunohistochemically for catecholamine-syntbesizing enzymes and neuropeptide Y [Halliday G. M. and McLachlan E. M. (1991) Neuroscience 43, 531–550] revealed that the majority of fluorescent neurons in both species probably contain dopamine-β-hydroxylase and neuropeptide Y as well as tyrosine hydroxylase. Those neurons lying just caudal to the facial nucleus immunoreactive for tyrosine hydroxylase and phenylethanolamine- N-ethyltransferase but not dopamine-β-hydroxylase and neuropeptide Y also lack catecholamine fluorescence. This rostral group of somata can be identified immunohistochemically in cats. The size and morphology of catecholamine-fluorescent neurons have been analysed in detail, and compared with the same features of the immunohistochemically stained neurons. Three morphological types of catechol- amine-containing neurons could be distinguished in material prepared by both techniques from rats and guinea-pigs, and in immunohistochemical material from cats. Rostral tyrosine hydroxylase-positive neurons, which differed morphologically from these three types, were present in all three species. On the basis of anatomical location, neuronal morphology and chemical characteristics, four groups of tyrosine hydroxylase-immunoreactive neurons have been identified in the ventrolateral medulla of rats, guinea-pigs and cats. Only the caudal three of these four groups appear to synthesize catecholamine, probably noradrenaline. From published data it seems likely that these four groups of tyrosine hydroxylase-positive neurons have distinct projections and functions related to cardiovascular and respiratory control.