Abstract
The recently proposed bacterial phylum Kiritimatiellaeota represents a globally distributed monophyletic clade distinct from other members of the Planctomycetes, Verrucomicrobia, and Chlamydiae (PVC) superphylum. Here, we present four phylogenetically distinct single-cell genome sequences from within the Kiritimatiellaeota lineage sampled from deep continental subsurface aquifer fluids of the Death Valley Regional Flow System in the United States.
Highlights
The recently proposed bacterial phylum Kiritimatiellaeota represents a globally distributed monophyletic clade distinct from other members of the Planctomycetes, Verrucomicrobia, and Chlamydiae (PVC) superphylum
Sequencing libraries were created for each single cell with the Nextera XT DNA library preparation kit (Illumina, San Diego, CA, USA) with the following modifications: purification was performed with column cleanup kits (Qiagen, Venlo, the Netherlands), and library selection was performed with BluePippin (Sage Science, Beverly, MA, USA) with a target sequence size of 500 Ϯ 50 bp
Raw sequencing reads for each single amplified genome (SAG) were quality trimmed with Trimmomatic v0.32 [8], reads with 95% or greater nucleotide identity with the Homo sapiens reference genome assembly (GRCh38) were removed, and low-complexity reads were removed as described previously [7]
Summary
The recently proposed bacterial phylum Kiritimatiellaeota represents a globally distributed monophyletic clade distinct from other members of the Planctomycetes, Verrucomicrobia, and Chlamydiae (PVC) superphylum. Raw water samples (1 ml) for single-cell genomics were amended with 5% glycerol and 1ϫ Tris-EDTA (TE) buffer (final concentrations), frozen on dry ice in the field, and stored at Ϫ80°C until cell sorting. Their genomes were amplified and sequenced at the Bigelow Laboratory for Ocean Sciences Single Cell Genomics Center as previously described [7].
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