Abstract
‘Touch’ or trace biological samples submitted to caseworking labs as evidence often contain biological material from multiple individuals which can result in mixed DNA profiles. These mixture profiles are difficult to interpret and may cause analytical bottlenecks for forensic laboratories. The data in this brief reports the variation in the relative abundance of intact epithelial cells deposited by four different donors across nine days. Touch samples were generated each day by rubbing a polypropylene tube with both hands for five minutes. Forward-scatter area (FSC-A) and side-scatter area (SSC-A) data was acquired with the BD FACSCanto™ II Analyzer. The relative abundance of different sub-populations within the FSC-A and SSC-A plots was calculated against the total number of events analyzed in each sample. Mean and standard deviation values were calculated for each donor.
Highlights
‘Touch’ or trace biological samples submitted to caseworking labs as evidence often contain biological material from multiple individuals which can result in mixed DNA profiles
Forward-scatter area (FSC-A) and side-scatter area (SSC-A) data was acquired with the BD FACSCantoTM II Analyzer
The relative abundance of different subpopulations within the FSC-A and SSC-A plots was calculated against the total number of events analyzed in each sample
Summary
Department of Forensic Science, Virginia Commonwealth University, USA article info Article history: Received 3 November 2015 Received in revised form 7 December 2015 Accepted 15 December 2015 Available online 19 December 2015 Keywords: Flow cytometry Forensic science Epithelial cells Touch DNA abstract ‘Touch’ or trace biological samples submitted to caseworking labs as evidence often contain biological material from multiple individuals which can result in mixed DNA profiles. These mixture profiles are difficult to interpret and may cause analytical bottlenecks for forensic laboratories. The data in this brief reports the variation in the relative abundance of intact epithelial cells deposited by four different donors across nine days. Touch samples were generated each day by rubbing a polypropylene tube with both hands for five minutes. Forward-scatter area (FSC-A) and side-scatter area (SSC-A) data was acquired with the BD FACSCantoTM II Analyzer. The relative abundance of different subpopulations within the FSC-A and SSC-A plots was calculated against the total number of events analyzed in each sample. Mean and standard deviation values were calculated for each donor. & 2015 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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