Forty-Hertz audiovisual stimulation does not have a promoting effect on visual threshold and visual spatial memory.

Abstract

Previous research has demonstrated that 40-Hz audiovisual stimulation can improve pathological conditions and promote cognitive function in mouse models of Alzheimer's disease. However, limited research has been conducted on humans, and the results have been inconsistent. In our study, we divided participants into an experimental group and a control group to investigate whether 40-Hz stimulation could enhance performance in visual threshold tasks and working memory task. In Experiment 1, we used a light bulb as the stimulus source and found a general practice effect, but no difference between the groups. In Experiment 2, we used a computer screen as the stimulus source and set the stimulation frequency to 48Hz. In Experiment 3 , we used a computer screen and audio as stimulus sources, simultaneously applying a 40-Hz stimulation to both visual and auditory modalities. Both experiments only revealed the disappearance of practice effects in the 40-Hz (48-Hz) group. Experiment 4 focused on testing visual spatial memory, but did not identify any significant differences between or within groups. In Experiment 5, we tested various visual spatial frequencies; yet again, no significant differences were found. Based on the comprehensive results, we conclude that a 40-Hz stimulation does not have a promoting effect on visual threshold or visual spatial memory.