Forty-five years of developmental biology of photosynthetic bacteria

Abstract

Developmental biology and cell differentiation of photosynthetic prokaryotes are less noticed fields than the showpieces of eukaryotes, e.g. Drosophila melanogaster. The large metabolic versatility of the facultative purple bacteria and their great capability to adapt to different ecological conditions, however, aroused the inquisitiveness to investigate the process of cell differentiation and to use these bacteria as model system to study structure, function and biosynthesis of the photosynthetic apparatus. The great progress in research in this field paved the way to study principal mechanisms of cellular organization and differentiation in these bacteria. In this article, the history of the research on membrane structure and development of anoxygenic photosynthetic prokaryotes during the last 45 years is described. A personal account of how I entered the field through research on the phototaxis of cyanobacteria is given. Intracytoplasmic membranes (ICM) were detected by electron microscopy in cyanobacteria and in purple non-sulfur bacteria. The formation of ICM by invagination of the cytoplasmic membrane in purple bacteria was observed for the first time. Investigations on the effect of changes in oxygen tension and light intensity on the formation of pigments and intracytoplasmic membranes followed. The isolation, purification, and analysis of light-harvesting complexes and of pigment-binding proteins was the next step of our research. Lipopolysaccharides and peptidoglycans were detected and analyzed in the outer membrane of photosynthetic bacteria. Functional membrane differentiation includes variations in the rates of photophosphorylation and electron transport. Molecular genetic approaches have initiated the investigation of transcriptional regulation and the analysis of correlation between pigment and protein synthesis. Molecular analysis of assembly of light-harvesting complexes and membrane differentiation are the present aspects of our research. Cell differentiation has been considered under evolutionary view.