Forty five minutes of ischemia and 24 h of reperfusion in the Wistar rat induce an acute pre‐renal injury which is not improved by 4 days of pre‐ischemia treatment with Spironolactone

Abstract

To characterize what kind of renal injury is induced by 45 min of ischemia and 24 h of reperfusion (I/R) and assess the effect of 4 days of pre‐ischemia treatment with Spironolactone (Sp = 20 mg/kg/day/o.g.gavage) or Vehicle (V = 560μl/day/o.g. gavage) on this I/R model, Wistar male rats (~250g of bw) were random assigned to three groups: V+Sham‐I/R, V+Real‐I/R & Sp+Real‐I/R and the following parameters were measured: glomerular filtration rate (GFR = CCr = ml/min/100g), urinary protein excretion (UPrV = mg/24h/100g), fractional excretion of sodium (FENa = %), free water clearance (CH20 = ml/min/100g). n GFR UPrV FENa CH2O V+Sham‐I/R 8 0.51±0.04 7.2±0.6 2.1±0.8 −2.0±3.3 V+Real‐I/R 8 0.28±0.03# 16.2±3.0° 2.7±1.2 −3.4±3.0 Sp+Real‐I/R 8 0.12±0.03& 17.3±3.0° 15.7±5.5°* −0.7±4.0 Values are mean ± SEM; °p< 0.05, p=0.01, p=0.0001 vs. V+Sham‐I/R; p<0.05, p<0.005 vs. V+Real‐I/R There were no differences among groups in 24h urinary nitrogen oxides excretion, renal malondialdehyde content or baseline renal superoxide production either.Conclusions45 min of ischemia and 24h of reperfusion in the Wistar rat induce an acute pre‐renal injury, which unexpectedly was not improved by 4 days of pre‐ischemia treatment with spironolactone. Supported by CO9‐FAI‐03‐20.20.