Formulations for Effective Detoxification Derived from Three Medicinal Plants: Thunbergia laurifolia, Clerodendrum disparifolium and Rotheca serrata.

Abstract

Based on the long history of the medicinal use of Thunbergia laurifolia, Clerodendrum disparifolium and Rotheca serrata, the extract formulations of these species: T. laurifolia and C. disparifolium; T. laurifolia and R. serrata; and T. laurifolia, C. disparifolium and R. serrata, called formulas 1, 2 and 3, were created for detoxification testing to take more advantage of each species. The objective of this study is to estimate the detoxifying effects of studied extract formulations on human cell and tissue culture as a preclinical trial. The major phytochemicals were derived by GC-MS. The detoxification efficacy of these formulations in cells and DNA levels were derived by MTT and comet assays in toxic PBMCs (incubated with rice whisky or bathroom cleaner). The phytochemical constituents were detected at 23.48% phytol and 43.03% oleamide in T. laurifolia; 12.88% oleamide, 20.93% 9,12,15-octadecatrien, 25.52% squalene, 22.19% butylated hydroxy toluene and 15.36% vitamin E in C. disparifolium; and 30.41% phytol, 32.78% oleamide, and 12.20%, 9,12,15-octadecatrien-1-ol in R. serrata. The toxic cells treated with the plant formulas 1, 2 and 3 showed no IC50 values, but formulas 1 and 2 displayed higher efficacies than formula 3 did. The comet assay indicated that the experiments (the treatment on toxic cells with the plant formulas) induced significant (p < 0.05) DNA damage compared to the negative control due to poisoning occurring before administration of the plant formulas. The OTM of the controls was significantly (p < 0.05) longer than the experimental samples showing significantly reduce the toxicity of the created formulations. The formulas showed high detoxification efficacies and formulations 1 and 2 resulted in higher levels of detoxification than formulation 3, especially in immediate treatment after receiving toxic substances.