Formulation, Standardization, and Preclinical Evaluation of Polyherbal Suspension against Inflammatory Bowel Disease

Abstract

The pharmacological healing for inflammatory bowel diseases continues to be uncertain and requires immediate therapeutic interventions. A poly-herbal formulation obtained from a traditional and authentic classic text of Ayurveda was assessed for its effect against IBD (inflammatory bowel disease) in this study. The formulated poly-herbal suspension comprises three different drugs namely, Burma dhaniya (Eryngium foetidum), Sapota (Manilkara zapota), and Curry leaves (Murraya koenigii). The formulated suspension was evaluated for certain standard parameters like organoleptic and accelerated stability studies at various temperatures. It was checked for its efficacy by oral route in acetic acid-induced colitis affected Balb/c mice. Mice were orally administered with formulated suspension (275 mg/kg, 550 mg/kg,), every 24 hours for 10 days. Histopathology, macroscopic damage score, myeloperoxidase (MPO) activity, and red blood cell parameters were evaluated after treatment. Reduction in the MPO activity, decrease in the macroscopic damage scores, and an increase in RBC cell count were seen distinctly at a high dose of 550 mg/Kg. The results obtained, established the effectiveness of the poly-herbal suspension against inflammatory bowel disease by treating the mice from acetic acid-induced colitis by reducing inflammation and oxidative damage to the colon. The maximum therapeutic effective activity was found to be 550 mg/kg for IBD mice.