Formulation of Trichoderma Based Biopesticide for Controlling Damping off Pathogen of Eggplant Seedling

Md Jannatul Adan,Afsana Jahan,Md Mahfuzar Rahman,Md Abdullahil Baque,Md Rafiqul Islam

doi:10.13189/ujar.2015.030305

Abstract

The effect of eight Trichoderma based substrates viz. rice bran, wheat bran, lentil bran, gram bran, black gram bran, mustard oil cake, grass pea bran and saw dust in mixing with peat soil and water were used in the formulation, these were evaluated for sporulations of Trichoderma harzianum and acting against Sclerotium rolfsii for the management of damping off of eggplant seedlings with control. The effect of the treatments varied significantly in terms of production of Trichoderma spore and reducing damping off and tip over increasing germination percentage, plant height, seedling vigor and fresh weight of eggplant seedlings in comparison to control. Among the treatments soil application with T5 (Trichoderma + Black gram bran + Peat soil + Water), T7 (Trichoderma + Grass Pea bran + Peat soil + Water) and T4 (Trichoderma + Gram bran + Peat soil + Water) showed the promising effect in controlling pre-emergence damping off, post-emergence damping off, tip over and increasing germination percentage, plant height, vigor index and fresh weight of seedlings. The highest germination percentage (78.00%) was observed in treatment T5 at 16 days after sowing (DAS). The lowest percentage (6.33%) post- emergence damping off was observed in T5 in eggplant seedling at 16 DAS. The lowest pre- emergence damping off and tip over were observed in T5 in eggplant (2.33% and 1.33%).

Highlights

The eggplant or brinjal (Solanum melongena L.) belongs the family Solanaceae and genus Solanum
According to the performance of the treatments the highest increase of seed germination (68.36%) was counted in T5 (Trichoderma + Black gram + Peat soil + Water) followed by T7 (56.85%) (Trichoderma + Grass pea bran + Peat soil + Water), T4 (51.10%) (Trichoderma + Gram bran + Peat soil + Water) and T2 (40.29%) (Trichoderma + Wheat bran + Peat soil + Water) respectively
In case of eggplant seedlings, the results revealed that the treatment T5 (Trichoderma + Black gram bran + Peat soil + Water) and T7 (Trichoderma + Grass Pea bran + Peat soil + Water) had remarkable effect against damping off and tip over of seedlings increasing the seed germination, seedling height, vigor index and fresh weight of seedlings irrespective of days after sowing (DAS)

Summary

Introduction

The eggplant or brinjal (Solanum melongena L.) belongs the family Solanaceae ( known as the Aubergine) and genus Solanum. The total area of brinjal cultivation was 78000 acres where 44000 acres was grown in Kharif season (summer) and 34000 acres in Rabi season (winter) with a total annual production of 246000 tons [2], which is very low in comparison to that of other countries like India, China, Egypt etc Such a potential crop is known to suffer from twelve diseases. Trichoderma produces chemicals called trichodermin which is responsible for its antagonistic properties [14]. T. harzianum may be used as an ecofriendly option to save many beneficial micro-organisms in the nature. This biocontrol agent would be potential to protect seedlings against diverse soil borne pathogenic fungi. Trichoderma harzianum CP bio-pesticide was prepared in peat soil based

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Conclusion