Formulation of New Media from Dairy and Brewery Wastes for a Sustainable Production of DHA-Rich Oil by Aurantiochytrium mangrovei.

Giovanni L Russo,Prospero Di Pierro,Maria Oliviero,Angela Sorrentino,Raffaele Sacchi,Beatriz Martín-García,Paolo Masi,Vito Verardo,Marco Baselice,Antonio L Langellotti

doi:10.3390/md20010039

Giovanni L Russo, Prospero Di Pierro + Show 8 more

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https://doi.org/10.3390/md20010039

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Abstract

Mozzarella stretching water (MSW) is a dairy effluent generated from mozzarella cheese production that does not have a real use and is destined to disposal, causing environmental problems and representing a high disposal cost for dairy producers. Spent brewery yeast (SBY) is another promising food waste produced after brewery manufacturing that could be recycled in new biotechnological processes. Aurantiochytrium mangrovei is an aquatic protist known as producer of bioactive lipids such as omega 3 long chain polyunsaturated fatty acids (ω3 LC-PUFA), in particular docosahexaenoic acid (DHA). In this work MSW and SBY have been used to formulate new sustainable growth media for A. mangrovei cultivation and production of DHA in an attempt to valorize these effluents. MSW required an enzymatic hydrolysis to enhance the biomass production. The new media obtained from hydrolysed MSW was also optimized using response surface methodologies, obtaining 10.14 g L−1 of biomass in optimized medium, with a DHA content of 1.21 g L−1.

Highlights

Long chain ω-3 polyunsaturated fatty acids (LC-PUFA) have a series of beneficial effects on human health [1]
In another work where Aurantiochytrium sp. was tested on a media supplemented with demineralized cheese whey (CW), the thraustochytrid showed significant growth only when supplemented with glycerol [8]
A previous study evaluated the growth of Schizochytrium sp. using lactic acid instead of glucose [25], and the authors reported that the biomass growth with lactic acid medium was lower than the glucose medium

Summary

Introduction

Long chain ω-3 polyunsaturated fatty acids (LC-PUFA) have a series of beneficial effects on human health [1]. Thraustochytrids, a heterotrophic funguslike clade of Stramenopiles, represent a potential alternative to fish oil due to their high biomass and DHA productivity, which is much higher than the fish source [6]. Aurantiochytrium (known as Schizochytrium until 2007) is a genus industrially exploited for the production of DHA [7]. Aurantiochytrium can produce high amounts of lipids (up to 55% of dry weight) and most of that is DHA (up to 35% of total fatty acids) [7]. New biotechnological processes based on the recycling of low cost side-streams from food industries would be an interesting way to produce omega-3 oil with lower production costs. Has been tested on different types of food waste, showing a high metabolic versatility to utilizing different type of organic and nitrogen sources [10,11] Aurantiochytrium sp. has been tested on different types of food waste, showing a high metabolic versatility to utilizing different type of organic and nitrogen sources [10,11]

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