Formulation of Methotrexate Loaded Solid Lipid Nanoparticles By Micro Emulsion Technique

Abstract

AIM: The aim of this study was to develop and characterize Methotrexate loaded solid lipid nanoparticles by Microemulsion technique. Background: Methotrexate is a preferable anti metabolite drug. It is used in the treatment of certain cancers like breast cancer, skin and lung cancer. Clinical studies have revealed that the curative effect of MTX tablet on cancers was limited due to their toxic dose-related side effects to normal cells, nephrotoxicity, and bone marrow suppression, acute and chronic hepatotoxicity and also due to the drug resistance of the tumour cells. Hence, there is a need to develop methotrexate solid lipid nanoparticles in order to minimize the adverse effects associated with the MTX tablet dosage form. Objective: The objective of the research work is to formulate, characterize and evaluate Methotrexate solid lipid nanoparticles by micro emulsification solidification technique. Methods: Solid lipid nanoparticles are prepared by using lipids stearic acid and glycerol monostearate by varying the concentration of surfactant. Three formulations were prepared with each lipid. Micro emulsion technique was adopted for the preparation of solid lipid nanoparticles. Each formulation was evaluated for drug content, entrapment efficiency, loading capacity& invitro drug release studies. Both the lipids were compared for the characterization and evaluation parameters. Result: On comparison Glycerol monostearate was found to be a better lipid over Stearic acid for the preparation of Methotrexate solid lipid nanoparticles because of its smaller mean particle diameter (238.8 nm), higher stability (-56.5 mV) and greater entrapment efficiency. Conclusion: Methotrexate solid lipid nanoparticles were successfully prepared with higher stability and drug release rate.