FORMULATION OF MEMORY SUPPORT TARGETED NANOSTRUCTURED LIPID CARRIERS (NLCS) LOADED WITH KELULUT HONEY EXTRACT PRODUCED WEST KALIMANTAN

Abstract

Objective: The purposes of this study were to find active compounds of the secondary metabolites, screen and determine the comparison of solid lipid, liquid lipid, and surfactant to produce the optimal NLCs formulation, analyze characteristic optimal NLCs loaded honey extract, and analyze the effectiveness of the optimal NLCs formulation as memory support in vitro. The Design-Expert software used for optimal NLCs kelulut honey extract formulation. Methods: The research method begins with the extraction process using ethanol solvent's maceration method. The formulation of NLCs begins with screening honey extracts for various solid lipids, liquid lipids, and surfactants. Next, the formulation design uses a D-optimal mixture design to obtain 14 run variations of glyceryl monostearate: tocopherol oil: tween 20 and evaluation using transmittance response, pH, and entrapment efficiency. The data on the response is entered into the software to obtain the optimal NLCs honey extract formula. The optimal NLCs honey extract were evaluated for transmittance, pH, entrapment efficiency, particle size, transmission electron microscope (TEM), Fourier transform Infrared Spectroscopy (FTIR), and in vitro activity as memory support. Results: Based on the evaluation, the water content of honey extract was 5.74 %±0.144; metals present in honey are Pb (0.003 mg/kg) and Cd (0.175 mg/kg). Phenolic and Total Flavonoids are 25.91 mg GAE/g±0.15 extract and 182.36 mg GAE/g extract±0.28. The optimal NLCs obtained combined glyceryl monostearate: tocopherol oil: tween 20 with a 0.5: 5.54: 23.96 composition ratio. The optimal NLCs kelulut honey extract formulation for transmittance value was 94.58%±1.54, pH was 6.59±0.15, and Entrapment efficiency was 99.89 %±0.09. Determination for particle size was 327 nm±0.57, and TEM and FTIR provided details on their structure. Evaluation for memory support in vitro, IC50 NLCs optimal formula 61.99±0.34; honey without extract 72.59±0.79; honey extract 38.55±0.24; and NLCs of base optimal formula without extract 829.81±0.93. The real-time stability shows optimal NLCs honey extract stable in real-time stability and freeze-thaw. Conclusion: NLCs from honey extract can be formulated from optimal NLCs using Design-Expert software. NLCs from honey extract has physical characteristics according to requirements and is stable. In vitro antioxidant studies revealed that the optimal formulation NLCs loaded honey extract had higher activity memory support with IC50 61.99±0.34.