FORMULATION OF ANTI ACNE CREAM CONTAINING NATURAL ANTIMICROBIALS

Abstract

This study investigate s about the production of nano - emulsion from coconut oil, lauric acid, and liposome containing lauric acid, xanthone, and na no gold using advanced technology . In addition, the antimicrobial effect of produced nano emulsion and nano gold was investigated. Using ultrasound it was pos sible to produce nano emulsion from lauric acid with oil droplet size of about 100 nm within 30 mi n utes at 45 ° C and 100 % amplitude. High pressure jet technique (at 3000 bars) was a suitable and fast method for production of nano emulsion using coconut oil. The droplet size of nano - em ulsion was nearly independent of nozzle size (OD = 80 to 100 μ m) and ranged from 185 to 240 nm. Production of gold nano - particle s using Turkevich’s method results in gold nan o - particles of about 115 nm to 1385 nm dependent on conc entration of gold solution. Atom Force Microscopy (AFM) confirmed the nano size of produced gol d nano particle s . In addition, the ultrasonic method was suitable to produce liposome from a mixture of lauric acid, xanthone and lecithin. The major parameter for droplet size of liposome was the concentration of lecithin in the sample. The optimum condit ion for liposome production using ultrasound was at 0.5 %, lecithin, 26 minute s and amplitude 90 % with average particle size of 116 nm. Xanthone concentration higher than 100 ppm was effective f or the growth inhibition of Propionibacterium acnes (Propioni bacterium acne) . This showed that lauric acid has inhibition effect on Propionibacterium acne as well.