Formulation Development and Evaluation of Polyherbal Mouthwash Containing Psidium Guajava L.

Abstract

The mouthwashes prepared from the herbal ingredients has shown great potential to overcome the chemical-based preparations. The combination of two or more herbal ingredients to form a polyherbal formulation has become an efficient approach to add value to the basic herbal formulation. In present study, the polyherbal mouthwash has been prepared using the leaves of Psidium guajava (guava), rhizomes of Curcuma longa (turmeric) and flower buds of Syzygium aromaticum (clove). All the three plant materials were collected and separately extracted. Phytochemical screening and quantitative estimation has been performed. The formulation has been prepared by optimization of different concentrations of used active ingredients. All the prepared formulations had undergone evaluation for organoleptic properties, pH and viscosity. The in-vitro antibacterial study has been performed by agar well diffusion method. The bacteria selected for the study were S. aureus, S. mutans and E. coli. The prepared mouthwash shows promising results in terms of zone of inhibition. When compared the prepared mouthwash with the marketed one, it shows greater zone of inhibition due to the synergistic effect produced by the multiple herbs used in the formulation. From the results obtained in this research work, it can be clearly stated that polyherbal mouthwash has become an effective way to improve the efficacy of herbal formulations. The combination of Psidium guajava (guava), Curcuma longa (turmeric) and Syzygium aromaticum (clove) in a mouthwash has shown promising results to improve the chances of usage of polyherbal mouthwash to commercially available chemical-based product.