Formulation-based antagonistic endophyte Amycolatopsis sp. SND-1 triggers defense response in Vigna radiata (L.) R. Wilczek. (Mung bean) against Cercospora leaf spot disease.

Abstract

In the present work, Amycolatopsissp. SND-1 (SND-1) was isolated fromCleome chellidoniiLinn. (C. chellidonii) was performed as biocontrol and resistance elicitor inVigna radiata(L.) R. Wilczek (mung bean) plants against Cercospora leaf spot causing pathogenCercospora canescens(C.canescens). The SND-1 isolate showed 74% of inhibition againstC. canescensin dual culture and GC-MS analysis revealed the presence of antifungal compounds. Molecular characterization through 16S rRNA showed that the isolated SND-1 belongs to Amycolatopsis sp. Thein vitroplant growth trials exhibited production of indole acetic acid, gibberellic acid, cytokinin, ammonia, hydrogen cyanide, and siderophore and phosphate solubilization. In vivostudy with talcum formulation of SND-1 revealed a significant increase in plant root length, shoots length, root and shoot fresh weight, and reduced the disease severity in treated mung bean plants. Triggering of resistance by SND-1 formulation was studied by histochemical depositions and biochemical defense enzymes that resulted in the acceleration in defense response in comparison with control plants. The bioactive endophyticAmycolatopsissp. SND-1 enhanced the defense againstC. canescensinfection; hence, it can be used as a biological control agent in mung bean cultivars.