Formulation and In vitro Cytotoxicity Studies of Avastin Nanoparticle Mediated Targeted Nose to Brain Drug Delivery System

Abstract

Nanoparticles (NPs) occupy a superior place among the recent approaches experimented to deliver drugs into brain by circumventing the BBB such as chemical modifications. The Emulsification PEG-PLGA nanoparticles carrying drug were made using the solvent evaporation method. In summary, PEG-PLGA was dissolved in acetone (50 mL) and PLGA was dissolved in PEG-PLGA (50:50, 7-11 mg/mL). The Chitosan-Avastin conjugates were created in the lab in accordance with the procedures and guidelines outlined in Chitosan (3.25 m mol) was hydrated in 1 N HCl, then the appropriate amount of distilled water was added. After adding NaOH (5N) to the reaction mixture to get the pH down to 5, Avastin (6.48 mmol) was added while the mixture was continuously stirred. In-vitro studies the basic aim is to check the effect of various drugs on various cancer cell lines. For testing purpose a large number of human cancer cell lines have been screened. Cells are cultivated in 96-well culture plates, and the rate of cell multiplication, which determines the rate of cell growth, is indirectly measured by the dye's color intensity, which is directly inversely proportional to the number of cells present. Using doses between 10 and 100 g/mL, the MTT test was performed to examine the cytotoxicity various polymer ratios and formulations on human lung cancer lines-A549. For the determination of the percentage of viability of various samples, the cancer cells without receiving therapy were utilized as the control.