Abstract
Objective: To analyze the effects of the topical gel that consists of quercetin nanoparticles on the mono iodoacetate-induced osteoarthritis.
 Methods: Injection of lecithin-quercetin into chitosan-tocopheryl polyethylene glycol succinate (TPGS) was used to prepared quercetin nanoparticle. Evaluation parameter for the quercetin nanoparticle including polydispersity index, zeta potential, particle size using particle size analyzer and morphology were executed using a transmission electron microscope. The evaluation of quercetin nanoparticle gel includes organoleptic and also a homogeneity of quercetin nanoparticle gel includes pH and viscosity. Quercetin nanoparticle gel was given for osteoarthritis (OA) animal model. The amount dosage of quercetin nanoparticle used in this study was 0.84, 1.68, and 3.37 mg/g gel. Measurement of the edema volume was conducted on day 7, 14, 21, 28, 35, 42, 49, 56, 63, and 70.
 Results: The quercetin nanoparticle gel dosage 1, 2, and 3 could reduce inflammation on two osteoarthritis animal models. On DMM group, 14 d after the treatment, there were significant differences between negative group and dosage 3 (P=0.028) and A. conyzoides extract gel (P=0.015). It has shown that injection of mono iodoacetate intraarticularly significantly increased the volume of edema in all groups that had been given by mono iodoacetate on day 7 to day 28. On the 42 d after administration of quercetin nanoparticle gel, there were significant differences between the normal and dosage 3 group (P= 0.000).
 Conclusion: Quercetin nanoparticle gel could reduce inflammation and prevent cartilago damage on histological evaluation.
Highlights
Osteoarthritis (OA) is a disease with several characteristics, including joint pain, definite motion, and inflammation without systemic effect
The lecithinquercetin solution was added into chitosan-tocopheryl polyethylene glycol succinate (TPGS) solution using plastic needle tubing with 1000 rpm agitation for 30 min
The yellow color of nanoparticle was achieved from the color of quercetin powder
Summary
Osteoarthritis (OA) is a disease with several characteristics, including joint pain, definite motion, and inflammation without systemic effect. Inflammation the key modulator on the pathology of osteoarthritis, inflammation could be the targeted therapy for osteoarthritis [1, 2]. Chondrocytes of knee osteoarthritis can increase the activity and production of inflammatory cytokines, which are made by other joint tissues [3]. The purpose of osteoarthritis therapy is to improve joint caused by inflammation and reduce patient, pain from daily activity. Pain aggressing joint can become worst because of joint motion. OA symptoms such as stiffness of joints, chronic pain, and muscle weakness, are the serious risk factor for patients with osteoarthritis with outcomes of impaired quality and mobility limitation [4]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
