Formulation and Evaluation of Polyherbal Antimicrobial Handwash

Abstract

Maintaining hand hygiene and cleanliness is crucial. Instead of relying on chemical agents, we formulated a polyherbal handwash using extracts from herbs with antimicrobial properties. Extracts from neem, turmeric, reetha, lemongrass, orange peel, and glycerin were used to prepare the handwash. The aim was to develop a more hygienic and reliable handwashing routine. The results demonstrated that the handwash prepared with methanol extracts exhibited greater antimicrobial activity compared to existing commercial handwashes. Stability studies showed no color change, phase separation, and the formulation retained its activity. The pH and viscosity were found to be 6.88 and 40-120 mPa.s, respectively. The antimicrobial efficacy of the developed polyherbal handwash was evaluated against skin pathogens such as Staphylococcus aureus and E. coli using the dip-well method. The efficacy of the handwash was validated, and the findings were compared with a commercially available handwash. The handwash formulation with lemon juice exhibited enhanced antimicrobial activity compared to the one without it. The combined action of phytoconstituents from the herbal extracts, known to be devoid of adverse effects on human tissues while promoting hygiene, rendered the desired antimicrobial effect to the polyherbal handwash formulation