Abstract
Objective: The purpose of this research was to formulate and evaluate the and anti-aging activity and the characteristic of liquid crystal emulsion of extra virgin olive oil (EVOO).
 Methods: Preparation of liquid crystal emulsion was made using the variation of alkyl polyglycoside and caprylic triglyceride as surfactant with concentration ratio was 3:5 (F1), 4:4 (F2), 5:3 (F3) and 5% extra virgin olive oil as an anti-aging substance. The liquid crystal emulsions were evaluated for an organoleptic test, microscopic, pH value, viscosity, particle size, zeta potential during 12 w storage at room temperature and centrifugation test. The selected liquid crystal emulsion was examined with anti-aging activity (moisture, pore, spot, and wrinkle) using a skin analyzer to volunteer’s skin.
 Results: The result showed that all formulas had stable storage for 12 w at room temperature (25±1 °C). Liquid crystal emulsion with a surfactant concentration of alkyl polyglycosides and caprylic triglycerides 4:4 (F2) showed the presence of liquid crystal, the stable value of pH (6.9) and viscosity (4995 mPa), smallest particle size 5.727 µm, and highest zeta potential-34.5 mV after 12 w storage at room temperature.
 Conclusion: EVOO liquid crystal emulsion (F2) showed better skin anti-aging activity (condition of moisture content, pore, spot, and wrinkle) than conventional emulsion. It concluded that the formula was stable physically and showed better skin anti-aging activity than conventional emulsion.
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