FORMULATION AND EVALUATION OF CREAM PREPARED FROM CROTON SPARSIFLORUS MORONG AND THEIR WOUND HEALING ACTIVITY

Abstract

Topical application of cream prepared from the chloroform extract of Croton sparsiflorus Morong were formulat ed and evaluated for i ts efficacy and safety. Creams we re prepared at different concentrations i.e. 5%, 7.5% and 10% (w/w) by fusion met hod using different excipients. These topical formulations were tested f or pH, viscosity, spreadability and drug content s uniformity. The stability study was carried out at 4, 25 and 37 0 C. The drug content uniformity of creams was found within the range of 98.09 to 98.21% . All the formulations were evaluated for its acute skin irritancy, wound healing activity in Swiss Alb ino rats. These formulations did not produce any skin irritation for about a week when applied over the skin. Comparative studies showed that the viscosity of the formulations increases, spreadability decreases and vice versa . From the stability studies, c reams showed no changes in properties after keeping at different temperatures for 90 days. All the formulations and the norma l chloroform extracts of C. sparsiflorus Morong showed significant (P<0.001) wound healing activity by excision wound model and com parable with that of the reference standards and control bases. The measurement of the wound areas were taken on 3rd, 6th, 9th, 12th, 15th and 18th days and the percentages of wound closures were calculated.