FORMULATION AND DEVELOPMENT OF GALLEN GUM LOADED SELF-ASSEMBLED MIXED MICELLES SYSTEM BASED ON FLAVONOID PHOSPHOLIPID COMPLEX

Abstract

Objective: Research on the development of pharmaceutical self-assembled mixed micells systems is in that they have the advantage of keeping the drug's encapsulating qualities while also enhancing its physicochemical characteristics. The goal of this study was to make the class II biopharmaceutical quercetin more soluble in water and more bioavailable when taken orally (QCT). The enhancement of encapsulation and flavonoids loading within mixed micelles using solvent evapouration technique. Methods: In the present study, pharmaceutical mixed micells of a BCS class II drug, QCT were prepared using solvent evapouration technique method. Prepared mixed micells were characterized using Critical micelle concentration (CMC), Fourier Transform Infrared (FT-IR), Particle size and zeta potential, Powder X-Ray Diffract meter (PXRD), In vitro dissolution, Transmission electron microscopy (TEM). In addition In vitro drug release studies were also performed. Results: The results of the characterization studies indicated the designing of gallen gum loaded self-assembled mixed micelles system based on flavonoid phospholipid complex. The CMC of LS-75 and LS-100 binary mixture had shows good results to be 0.0013%. The FTIR spectra of complex showed characteristic peak of QCT shows abundant effect on O-H (aromatic), C-O (aromatic), C-C, and aromatic C-O is observed at 3282.2, 1620.1, 1058.7, and 1162.2 respectively. The average particle size of design-optimized quercetin mixed micells (QCT-MMs) was demonstrated to be ~116.1 nm, as evaluated by Malvern. From the obtained particle size, it indicated that the particle size of QCT in QCT-MMs was widely distributed. The polydispersity index (PDI) for QCT-MMs was found in the range of ~ 1.000, zeta potential value for QCT-MMs as evaluated by Malvern was observed to be ~-99.2 mV. The P-XRD, SEM, showed good powder diffraction results with having good flow property. Also formulation were evaluated for the In vitro drug dissolution study for rate of extent of drug release and dissolution rate release of QCT from QCT-MMs was sustained up to 72 h. TEM images of QCT-MMs, where the micelles exhibited relatively regular dark stained shapes appearing more or less spherical or spheroid. Conclusion: It can be concluded that the QCT-MMs enhance the aqueous solubility of the QCT and increased the bioavailability and retention time.