Abstract
Objective: The study was aimed to extract, formulate and characterize collagen extract of Stichopus horrens into serum preparations and decide antioxidant activity in powders and serum preparations.
 Methods: The sea cucumber meat was extracted collagen in three stages, namely the pretreatment stage using 0.3 M NaOH solution 1:10 (w/v) for 48 h, the hydrolysis step in the 0.3 M 1:10 acetic acid solution (w/v) for 48 h, and the extraction stage with distilled water 1: 2 (w/v) for 2 h at 45 °C. The collagen extract was freeze-dried to obtain collagen powder. Collagen powder was characterized by HPLC and its antioxidant activity was determined using the DPPH method. Collagen powder formulated with extract variation of 0, 0.5 and 1%. Evaluation of serum included organoleptic, homogeneity, stability of pH and viscosity as well as antioxidant activity.
 Results: The results showed that collagen powder had a % yield of 0.24%, which consisted of the amino acids glycine, proline, alanine, and glutamic acid as the dominant amino acids. The % Free radical inhibition of collagen powder at concentration of 5000 ppm was 63.23%. IC50 values were obtained at 4045.37 ppm. The stability test resulted in stable serum preparations without significant changes at 4 °C and 27 °C±2 °C storage temperatures.
 Conclusion: The measurement of DPPH Radical reduction activity in the highest serum preparation was 1% extract with a value of 2.4%.
Highlights
The use of medicinal ingredients is derived from plants but marine life is being developed because it has great potential as a source of medicinal ingredients
The higher concentration of Stichopus horrens sea cucumber collagen extract resulted in a high percentage of free radical inhibition
In the extract of Stichopus horrens sea cucumber collagen based on the physical properties of collagen, the color of Stichopus horrens sea cucumber collagen is colorless after freeze dry, the color changes to brownish-white and has a cotton-like texture and has a pH of 6 according to the quality requirements of SNI 8076-2014 collagen, which has a pH of 6-7.5
Summary
The use of medicinal ingredients is derived from plants but marine life is being developed because it has great potential as a source of medicinal ingredients. Sea cucumbers are one of the most potential marine resources. 53 species of sea cucumbers are found in Indonesia, there are 22 species that can be consumed, and 8 of them have a high market value. Sea cucumbers have complete nutritional content due to often being referred as seabed ginseng and are used as supplements. The results of laboratory analysis showed that the dried sea cucumber extract contained up to 86.8% protein, 80% collagen, minerals, mucopolysaccharides, glucosaminoglycans (GAG), natural antiseptics, chondroitin, omega 3, omega 6, and omega 9, as well as various amino acids [2]
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