Abstract
Nanotherapeutics can enhance the characteristics of drugs, such as rapid systemic clearance and systemic toxicities. Polymeric nanoparticles (PRNPs) depend on dispersion of a drug in an amorphous state in a polymer matrix. PRNPs are capable of delivering drugs and improving their safety. The primary goal of this study is to formulate doxycycline-loaded PRNPs by applying the nanoprecipitation method. Eudragit S100 (ES100) (for DOX-PRNP1) and hydroxypropyl methyl cellulose phthalate HP55 (for DOX-PRNP2) were tested as the drug carrying polymers and the DOX-PRNP2 showed better characteristics and drug release % and was hence selected to be tested in the biological study. Six different experimental groups were formed from sixty male albino mice. 1,2,-Dimethylhydrazine was used for 16 weeks to induce experimental colon cancer. We compared the oral administration of DOX-PRNP2 in doses of 5 and 10 mg/kg with the free drug. Results indicated that DOX-PRNP2 had greater antitumor activity, as evidenced by an improved histopathological picture for colon specimens as well as a decrease in the tumor scores. In addition, when compared to free DOX, the DOX-PRNP2 reduced the angiogenic indicators VEGD and CD31 to a greater extent. Collectively, the findings demonstrated that formulating DOX in PRNPs was useful in enhancing antitumor activity and can be used in other models of cancers to verify their efficacy and compatibility with our study.
Highlights
Nanotherapeutics is a developing field within the field of nanomedicine and it aims to enhance the characteristics of drugs [1]
The primary goal of this study is to formulate pH-sensitive PRNPs loaded with DOX using the nanoprecipitation technique and the polymers eudragit S100 (ES100) or hydroxypropyl methyl cellulose phthalate HP55 (HPMCP HP55) and to test their therapeutic potential against experimentally induced colon cancer in mice
3 show that particle size of formulated nanoparticles were below micrometers and showed moderate stability owing to values of zeta potential (ZP)
Summary
Nanotherapeutics is a developing field within the field of nanomedicine and it aims to enhance the characteristics of drugs [1]. There are various types of nanotherapeutics such as solid-lipid nanoparticles, gold nanoparticles and polymeric nanoparticles (PRNPs) [2–5]. PRNPs improve solubility, bioavailability and retention time of drugs and enhance their therapeutic effect [6–9]. PRNPs have advantages over conventional drugs in that they increase drug efficacy, protect the drug from degradation when in contact with biological fluids and control the drug release [10] due to their advantages resulting from their tiny size. PRNPs have gained a lot of attention in recent years [11,12]. PRNPs are composed of amorphous drugs dispersed within a polymer matrix [13]. PRNPs can be utilized to be loaded with various types of medications for treatment of different types of cancer [16,17]
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