Abstract

There have been many studies on single strains in wastewater treatment and a new synthetic microbial community was prepared in this study, which provides a reference for the application of heterotrophic nitrification-aerobic denitrification in actual wastewater treatment. The growth period distribution of the composite bacteria was determined by plotting growth curves with different sole nitrogen sources, and the influence of the carbon source, carbon to nitrogen ratio (C/N) ratio, pH, and temperature on ammonia removal by the composite heterotrophic nitrifying-aerobic denitrifying strain was investigated. The optimal conditions for the heterotrophic nitrification process were sodium citrate as the carbon source, a C/N ratio of 10, a pH of 7, and a temperature of 30 °C, and only trace amounts of nitrate and nitrite were observed during the process. When the sequencing batch reactor (SBR) of a pig farm wastewater treatment plant was inoculated with the synthetic microbial community, the average removals of the chemical oxygen demand (COD) and ammonia nitrogen in the effluent were 92.61% and 20.56%, respectively. From the results, the synthetic microbial community was able to simultaneously perform heterotrophic nitrification-aerobic denitrification indicating great potential for full-scale applications.

Highlights

  • In recent years, the livestock and poultry breeding industry has gradually moved towards specialized and large-scale centralized feeding methods [1]

  • The purpose of this study was to develop an ammonia nitrogen degradation composite composed of heterotrophic nitrification-aerobic denitrifying strains, determine its reaction characteristics and its application in livestock wastewater treatment, and provide an experimental basis and theoretical support for future applications

  • Bacterial growth was monitored by monitoring the optical density at 600 nm (OD600 ) using a spectrophotometer

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Summary

Introduction

The livestock and poultry breeding industry has gradually moved towards specialized and large-scale centralized feeding methods [1]. Compared with traditional distributed breeding, large-scale breeding can significantly improve production efficiency, reduce production costs, and increase economic benefits. The development of large-scale farms has led to an increase in the amount of livestock and poultry manure runoff, which has placed tremendous pressure on the ecological water environment. Livestock and poultry aquaculture wastewaters contain large amounts of suspended solids, COD, nitrogen, and phosphorus, which cause the eutrophication of water bodies and water pollution. In China, the COD and total nitrogen from the livestock wastewater account for. 96% and 38% of the total agricultural wastewater, respectively [2]. Livestock and poultry wastewaters are not a commonly considered pollutant but contain

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