FORMULATION AND CARACTERIZATION OF QUERCETIN NIOSOME WITH CONCENTRATION VARIATIONS OF SPAN 20 SURFACTANT

Weka Sidha Bhagawan,Rahmi Annisa,Atiza Fajrin Maulidya

doi:10.24071/jpsc.002839

Abstract

Quercetin has low solubility, absorption and bioavailability which limits its practical use as a drug or supplement. Therefore, it is important to formulate a quercetin niosome system with various concentrations of span 20 as a surfactant. This investigation aimed to formulate and analyse a quercetin niosome preparation with span 20 variations to provide optimal quercetin solubility. Niosomes were prepared using various concentrations of span 20. In the present study, the quercetin niosome used the reverse phase evaporation (RPE) method. Quercetin niosome is characterised by its organoleptic properties, pH value, particle morphology comprising the particle shape and size, and encapsulation efficiency. Organoleptic observations of the quercetin niosome included a yellow colour, distinctive quercetin odour and thick consistency for all formulas. The pH remained within the physiological pH range of skin. Quercetin niosome morphology was close to spherical while the niosome particle size results were 2.13 µm (F1), 2.99 µm (F2) and 3.31 µm (F3). The quercetin niosome encapsulation efficiency results were 81.86 ± 0.47% (F1), 84.02 ± 0.26% (F2) and 88.24 ± 0.10% (F3). Quercetin niosome were successfully prepared using multiple span 20 concentrations below the cholesterol concentration characterised by the measurement results of organoleptic, pH, particle morphology and encapsulation efficiency.

Highlights

Quercetin (3,3′, 4′, 5, 7-pentahydroxyflavone) is a naturally occurring flavonoid compound found in many vegetables, fruits and nuts
The instruments used in this research included a 510 type pH meter by Eutech Instruments from Singapore, Hei-VAP core rotary evaporator by Heidolph from Germany, UV-1601 spectrophotometer by Shimadzu from Japan, FLEXSEM 100 scanning electron microscope (SEM) by Hitachi from Japan, S10H ultrasonic cleaner by Elma from Germany, magnetic stirrer by Mettler Toledo from Germany, and other glassware
Chloroform was used as a solvent for cholesterol, and distilled water was used as a solvent for quercetin while phosphate-buffered saline was in a liquid phase

Summary

Introduction

Pentahydroxyflavone) is a naturally occurring flavonoid compound found in many vegetables, fruits and nuts. These compounds are found in apples, onions, tomatoes, broccoli, lettuce, black tea and green tea (Patel et al, 2018; Rothwell et al, 2013). A large number of important pharmacological activities of quercetin have been identified in recent years. Quercetin has been reported to exhibit high antioxidant, antitumor, antiinflammatory, antimicrobial, antibacterial and antiviral activity (D’Andrea, 2015; Gonta et al, 2020; Lesjak, 2018; Lin & Zhou, 2018; Pal & Tripathi, 2020a, 2020b, 2019; Wang et al, 2016; Xu et al, 2019). It has been reported to exhibit antithrombotic, anti-aggregator and vasodilating activity (Chondrogianni et al, 2010; Chopra et al, 2000; Erlund et al, 2000)

Methods

Results

Conclusion