Abstract
Liquid soap is a liquid form that serves to cleanse the skin. The aim of this research are to know the optimization formulation and evaluation of liquid soap extract ethanol okra ( ) and also find the best concentration of okra extract as the inhibitors against Staphylococcus . Experimental laboratory was chosen as the research design. Liquid soap consists of 5 formulations, namely formulation 1, 2, 3, 4, and 5 which consist of 0%, 25%, 50%, 75% and 100% okra concentration. The antibacterial effectiveness was tested by disc method against liquid soap extract ethanol okra ( ) and the clear zone measured. The analysis done with two-way test. The results of the evaluation of liquid soap quality was appropriate with the standard of soap quality established by SNI 1994. The best moisture value found in the formulation of 5 (F5) with 0%form of 3.54%, the best free fatty acid found in formulation 4 (F4) with a concentration of 50% of 0.07135%, the best free alkali concentration was found in formulation 2 (F2) with a 25% concentration of 0.04194%, the best pH value was presented at concentrations of 0%, 25%, and 50% with a value of 9, the best value of foam stability found in Formula 1 (F1) with a 100% concentration of 9.1, the best density (BJ) test found in Formula 4 (F4) with 0% concentration. The result of ANOVA test with variation of ethanol okra concentration obtained p-value = 0.000 l0.05 which states there was a significant difference where liquid soap extract ethanol okra has antibacterial to Staphylococcus . The best inhibitory zone test resulted in formation 5 (F5) of 14.7 mm at 100% concentration.
Highlights
D-IV Teknologi Laboratorium Medis, Fakultas Ilmu Kesehatan, Universitas Muhammadiyah Sidoarjo, Jl
The aim of this research are to know the optimization formulation and evaluation of liquid soap extract ethanol okra (Abelmoshus esculentus) and find the best concentration of okra extract as the inhibitors against Staphylococcus epidermidis
Liquid soap consists of 5 formulations, namely formulation 1, 2, 3, 4, and 5 which consist of 0%, 25%, 50%, 75% and 100% okra concentration
Summary
Formulasi dan Evaluasi Sabun formulasi 1, 2, 3, 4, dan 5 yang masing-masing terdiri dari konsentrasi okra sebesar 0%, 25%, 50%, 75% dan 100%. Berdasarkan uraian di atas, maka dilakukan penelitian tentang formulasi dan evaluasi sabun cair ekstrak etanol Okra (Abelmoschus esculentus) serta dilakukan uji antibakteri terhadap Staphylococcus epidermidis. Yang dibutuhkan dalam penelitian ini adalah: simplisia buah Okra (Abelmoschus esculentus) yang diperoleh dari salah satu pasar Waru Sidoarjo, biakan bakteri Staphylococcus epidermidis diperoleh dari BBLK (Balai Besar Laboratorium Kesehatan) Surabaya, minyak biji bunga matahari, media NAS (natrium agar slaint) (p.a; Merck), media BAP (Blood Agar Plate) (p.a; Merck), darah merah domba 6%, phisiological zouth (PZ) steril, standar mac-farland, media mueller hinton (p.a; Oxoid), swap, paper disk antibiotik klorampenikol, blank paperdisk, asam stearat (p.a; Merck), gliserin (p.a; Emsure), KOH (p.a; Emsure), aquadest, alkohol (teknis) 70%, etanol (teknis) 96%, sukrosa (p.a; Merck), NaCl (p.a; Emsure), NaOH (p.a; Merck) serbuk Mg (p.a; Merck), kloroform (p.a; Emsure), HCl (p.a; Merck), FeCl3 5% (p.a; Merck), asam sulfat pekat (p.a; Emsure), dan indikator phenolphtalein (PP).
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