Abstract
The usefulness of o- nitrophenyl-β- D- galactopyranoside-urease-indole (ONPG-urease-indole medium, β-GUI), coupled with triple sugar iron agar (TSI), Kligler iron agar (KIA) and lysine iron agar (LIA), respectively, for the early detection of Salmonella, Shigella and Yersinia was evaluated and compared with the LIA combined with both TSI and KIA. The study was performed on 749 selected, lactose-negative Enterobacteriaceae isolates. The most effective combination was LIA-β-GUI, which showed the highest efficiency for Shigella Yersinia presumptive recognition. It also yielded good results for Salmonella, leading to the further examination of only four cultures more than TSI-β-GUI which was the best for Salmonella screening. Moreover, out of the 749 lactose-negative isolates, it considered as a ‘suspicious enteric pathogen’ the lowest number of strains (452, 60.3%). Out of these, 416 (55.5% of the total strains and 92.0% of those yet to be tested) actually belonged to Salmonella, Shigella or Yersinia genus.
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