Formula omitted] and aspartate raise the cytosolic freee calcium concentration by acting upon receptors transiently expressed on immature cerebellar Purkinje cells

Abstract

N- Methyl- d-aspartate (NMDA) or aspartate (Asp) increased the cytosolic free calcium concentration ([Ca] in) in some populations of Purkinje cells dissociated from immature rat cerebellum. The NMDA- and Asp-induced rise in [Ca] in was affected only a little by adding glycine or NMDA antagonists, but was reduced either by adding Mg 2+, Gallopamil hydrochloride (D-600) and γ-amino-butyric acid, or by removing external Na +. The results suggest that stimulation of the NMDA-sensitive receptors transiently expressed on immature Purkinje cell soma results in a rise in [Ca] in through the activation of voltage-dependend Ca 2+ channels.