Formation of vascular mimicry in human gastric cancer and the corelation with LOX and MMP-9

Abstract

Event Abstract Back to Event Formation of vascular mimicry in human gastric cancer and the corelation with LOX and MMP-9 Mei Han1*, Yang Cao1, Huanyu Sun1, Ru Bai1 and Wenjun Yang1 1 Ningxia Medical University, China To explore the corelations of lysyl oxidase(LOX) and matrix metalloproteinases-9 (MMP-9) with formation of VM in gastric carcinoma tissue. Gastric cancer tissues were collected from 49 patients with diagnosis of gastric cancer. According to metastasis (include lymph node and distant metastasis), gastric cancer tissues were divided into the group of metastasis and the group of non-metastasis. According to the invasion, gastric cancer tissues were divided into the group of T1 + T2 and the group of T3 + T4. The formations of VM were measured through CD34 and PAS double staining on gastric cancer tissue slices. The distributions of LOX and MMP-9 were observed using immunohistochemistry. Relative levels of LOX and MMP-9 were surveyed by western-blot and immunohistochemistry. The locations of LOX and MMP-9 were detected by Immunofluorescence. Results showed, in gastric carcinoma tissues, the VM formation in group with metastasis were more than that in group without metastasis(P<0.05), the level of LOX and MMP-9 protein were higher in group with metastasis than that in group without metastasis(P<0.05); the expression of LOX in group of T3 + T4 were more abundant than in T1 + T2 group(P <0.05). The levels of LOX ​​was positively correlated with VM formation in gastric carcinoma tissues, the levels of MMP-9 ​​was positively correlated with VM formation. The immunofluorescence results suggested that LOX often located in the area that MMP-9 expressed. The results suggested that VM promote the invasion and metastasis of gastric cancer, LOX and MMP-9 may be the facilitative factors of VM formation. Acknowledgements This research is support by national natural science foundation of china(81260314). Keywords: lysyl oxidase, vascular mimicry, gastric cancer, matrix metalloproteinase-9, metastasis, invasion Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Han M, Cao Y, Sun H, Bai R and Yang W (2013). Formation of vascular mimicry in human gastric cancer and the corelation with LOX and MMP-9. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00157 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 10 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Prof. Mei Han, Ningxia Medical University, Yinchuan, Ningxia, Ningxia Hui Autonomous Region, 750004, China, hanmei0708@126.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Mei Han Yang Cao Huanyu Sun Ru Bai Wenjun Yang Google Mei Han Yang Cao Huanyu Sun Ru Bai Wenjun Yang Google Scholar Mei Han Yang Cao Huanyu Sun Ru Bai Wenjun Yang PubMed Mei Han Yang Cao Huanyu Sun Ru Bai Wenjun Yang Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.