Abstract
Microtubule organization in dividing guard mother cells ofAllium cepa was investigated using tubulin immunofluorescence, in order to determine when the spindle assumes its characteristic oblique orientation. The prophase spindle is transverse, perpendicular to the preprophase band and the future cell plate. Its orientation then shifts, until the majority of spindles are oblique by late metaphase. These observations show that the early spindle conforms to positional information determining the division plane, but becomes oblique during later stages of division, perhaps in response to space constraints.
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