FORMATION OF THE MULTIPLE GRAPE SHOOTS IN CULTURE IN VITRO ON DIFFERENT NOURISHING MEDIA

Abstract

Semi-liquid nutrient media were used for the multiple grape shoots induction. Aim of the research was to study the agar concentration effect in different nutrient media and define influence of the media consistency on the survivability parameters, growth and development of initial explants, and the multiple grape shoots induction. Methods. Initiation of culture in vitro formation from explants, microclones cultivation, and the method of the meristematic plant regeneration were used. Nutrient media were liquid (without agar as a component), semiliquid (with agar content of 4 g/l), and solid (with agar content of 8 g/l), including an addition of 1 mg/l 6-BAP to all experimental media. The registration of the explants’ survivability, time of the beginning of axillary buds proliferation, and amount of obtained shoots was conducted. Results. Semi-liquid nutrient media were used. The advantage of using semi-liquid media in comparison with solid and liquid nutrient media is revealed. The search of the optimal nutrient medium for multiple grape shoots induction in vitro was successfully done. Modified semiliquid MS was determined as the optimal nutrient media. Its application contributed to better survivability, differentiation, and regeneration of grape meristems. On average in sortes, it formed 9.59 pcs., shoots, which further increases the coefficient of grape multiplication in culture in vitro to 1: 9. Conclusions. The application of the improved method of induction of multiple shoots contributed to a better survivability of the initial explants of grapes of technical varieties in culture in vitro, accelerates the processes of proliferation of the axillary microbuds, and the processes of generating more shoots suitable for subsequent clonal micropropagation.