Formation of the cranial motor neurons in the absence of the floor plate

Abstract

The inductive signals for the differentiation of motor neurons in the spinal cord have been experimentally shown to arise from cells in the midventral region of the neural tube, often referred to as the floor plate, and from the notochord. Although the prevailing view is that a similar mechanism accounts for the differentiation of motor neurons in the brain stem, supporting experimental evidence is lacking. Here, using the formation of the trochlear nucleus in the midbrain of duck embryos as a model system, we report that the floor plate and the notochord are not necessary for the development of these motor neurons in the brain stem. Early damage to the floor plate or extirpation of the floor plate and notochord does not prevent the development of these cranial motor neurons. Thus, either the inductive signals for the formation of these cranial motor neurons arise from some other structure or the germinal epithelium of the cranial neural tube is intrinsically programmed to form specific cranial motor nuclei.