Abstract
Pyrazines generated from aqueous sugar-glutamine model systems heated at 90°C were investigated quantitatively. To determine trace levels of pyrazines in aqueous matrices, an efficient method using solid-phase extraction (SPE) and gas chromatography/mass spectrometry (GCMS) was developed. A series of alkylpyrazines as well as trace levels of acetylpyrazines and bis-(2-furyl)pyrazines were detected in the model systems by this method. Remarkable difference in the formation of pyrazines between monosaccharide and disaccharide was observed. The yield of acetylpyrazines and bis-(2-furyl)pyrazines from maltose was larger than those from fructose and glucose, while that of alkylpyrazines was less. The pH dependency on the generation of pyrazines in maltose-glutamine model systems was also examined.
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