Formation of plaques by adherent spleen cells from nonimmunized mice, incubated in vitro with the nonadherent fraction of immune allogeneic cells.

Abstract

Twenty-four-hour incubation of adherent spleen cells from normal C57BL mice with purified nonadherent spleen cells from CBA mice, in the peak of their primary response against sheep red blood cells, resulted in the appearance of plaque-forming cells in the adherent cell population. The immunological nature of these plaques was demonstrated by their complement dependency and the inhibitory effect of specific anti-mouse IgM serum. Direct, but not indirect plaque formation was found to be associated with the adherent cell population for which direct cell to cell contact is necessary. Experiments with cytotoxic specific anti-strain sera revealed that in this system, the adherent plaque-forming cells are the nonimmune adherent C57BL cells.