Abstract
The crystalline fullerene C60 particles were formed and immobilized on poly(dimethylsiloxane) (PDMS) substrates under the various discharge conditions by an ink-jet method, and investigated for the reactive oxygen species (ROS) generation property under visible light irradiation. The particles were synthesized by discharging a toluene solution dissolved C60 and poly(methyl methacrylate) (PMMA) with the ink-jet spotting system. The ROS generation was evaluated by comparisons of the fluorescence intensities measured for the formed particles under green laser irradiation and in a dark room using fluorescent dyes, 2’,7’-dichlorofluorescein diacetate and dihydroethidium. The results of transmission electron microscope (TEM) observation showed that the formed particles consisted of crystalline C60. The optimal ink-jet discharge conditions for synthesizing the particles to generate more ROS were found. In the case of the optimal conditions, the structure in which the needle-like particles were three-dimensionally formed was confirmed. The surface area of the crystalline C60 particles was calculated using the SEM observation results, and it was suggested that when the needle-like finer particles were three-dimensionally formed under the optimal conditions, increasing the surface area lead to an increase in the ROS generation amount.
Highlights
Fullerenes, carbon allotropes, represent the unique physical and chemical properties, and have incited a considerable hope of their potential for uses in biomedical application
The surface area of the crystalline C60 particles was calculated using the scanning electron microscope (SEM) observation results, and it was suggested that when the needle-like finer particles were three-dimensionally formed under the optimal conditions, increasing the surface area lead to an increase in the reactive oxygen species (ROS) generation amount
The crystalline C60 particles were formed on the PDMS substrates under the various discharge conditions by the ink-jet spotting system, and investigated for the relationships among the conditions, the shape and the structure of particles, and the ROS generation
Summary
Fullerenes, carbon allotropes, represent the unique physical and chemical properties, and have incited a considerable hope of their potential for uses in biomedical application. By Markovic and coworkers, the cytotoxicity/ROS production of different nC60 suspensions prepared using tetrahydrofuran, ethanol and water were examined for investigating the mechanisms of the cytotoxicity [3] In this way, many studies on ROS generation by C60 have been performed using solutions with colloidal dispersions of C60. We have up to date studied an method to immobilize C60 at the specific locations on the surface of a polymer substrate, for the purpose of applying the photosensitizing properties of C60 as the function of microfluidic and microarray chips, called μ-TAS (micro total analysis systems). The effects of the discharge conditions and the structure of formed particles on the ROS generation were investigated [9]
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