Abstract
The replication of influenza virus in two strains of ascites tumor cells, Ehrlich's and Krebs 2, in vitro and in the mouse peritoneum was compared with growth of this virus in roller tube suspensions of minced chorioallantoic membrane. Extended incubation of tumor cells with influenza or Newcastle viruses resulted in a progressive cell destruction which was related in degree to the size of the inoculum. Titration of extracts from frozen and thawed tumor cells revealed replication of hemagglutinins which was especially marked in tumors in the mouse peritoneum, where there was little or no increase in infectivity titer. The formation of hemagglutinins in Ehrlich tumor cells was not appreciably diminished by anaerobiosis, but was slightly decreased by hypotonic medium or KCN. In roller tube suspensions of Krebs 2 cells, glutamine and lactate as substrates gave a higher yield of intracellular hemagglutinins than did glutamine and glucose. With the glucose medium, exclusion of oxygen favored the growth of virus in K2 cells.
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