Formation of Near-Infrared Fluorescent Nanoparticles for Medical Imaging

Abstract

Indocyanine green (ICG) is a US FDA-approved near-infrared fluorescent, water-soluble dye used for diagnostics in vitro and in vivo. The aim of this study was to develop insoluble nanoparticles based on a cationic polymer, ICG and a targeting molecule. The particles are intended for oral administration in the colon, having fluorescence in near-infrared, thus enabling remote detection. An aqueous dispersion of particles formed from Eudragit-RS by simple precipitation method possessing a mean size of approximately 100 nm and zeta potential of +16 mV was produced. These particles are capable of binding both ICG and fluorescein isothiocyanate-IgG via noncovalent interactions. These composite particles retain the emission characteristics of the fluorescent precursors and also exhibit potential specific recognition ability. The particles were stable in intestinal fluid and are composed only of materials that are FDA approved. The nanoparticles may be suitable for in vivo imaging and therapy by oral delivery systems.