Abstract
In laboratory experiments, two fungi that commonly colonize leaves of Spartina aZterniJEoru(L oisel.) were studied to determine if they contribute to the formation of humic substances, which have been isolated earlier from salt marsh estuaries. In incubations of up to one year in duration, Phaeosphaeria spartinicola and Phaeosphaeria halima did not form dark pigments when grown in an artificial, full-nutrient medium. However, if the nutrient solution was enriched with a water extract of S. alterniJEoro, appreciable amounts of dark brown substances were produced. Elemental (C, H, N, 0) and spectral (ultraviolet, visible, and Fourier Transform Infrared) analyses of these dark brown substances show them to be similar to salt marsh humic substances.
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