Abstract
In fermentation under anaerobic conditions with Saccharomyces cerevisiae, small quantities of esters (ethyl acetate) are formed. The formation of ethyl acetate has been studied in a synthetic medium with glucose as carbon source in fermentation with brewer's yeast (top yeast). Fermentations to which acetic acid and ethyl acetate respectively had been added, as well as kinetic studies on the chemical formation and hydrolysis of ethyl acetate, showed ethyl acetate to be formed within the cell according to an unknown reaction mechanism and not through the esterification of acetic acid by means of ethanol in the medium. The pH optimum for the formation of ethyl acetate was 4.4-4.6.
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