Abstract
Abstract Escherichia coli lysogenic for λ was superinfected with radioactive λ and the covalently closed DNA circles were studied by zonal centrifugation in an alkaline sucrose gradient. A fraction sedimenting more rapidly than monomer circles was observed. By a variety of tests these were shown to be mostly circular dimers; a small fraction of catenated dimers was also seen. Both structures are probably formed by joining of cohesive ends. The possible significance of these structures in genetic recombination is discussed.
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