Abstract
The formation of concatemers in T7 wild type- and amber 3 mutant-infected bacteria begins soon after the completion of the first rounds of replication. Unreplicated viral DNA is not incorporated into concatemers. Parental DNA strands are recovered as intact unit-length molecules from concatemers in gene 3 mutant-infected cells. In concatemers from wild type-infected cells a considerable fraction of parental DNA sequences is covalently linked to newly synthesized DNA sequences probably due to molecular recombination. No concatemeric DNA is formed in the absence of a functional gene 6 product (exonuclease) although several rounds of DNA replication occur under these conditions. The replicated gene 6 mutant DNA is partially fragmented.
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