Formation of complement split products and proinflammatory cytokines by reinfusion of shed autologous blood

Abstract

1. The purpose of this study was to determine whether shed autologous blood collected postoperatively contains complement split products (C3a and SC5b-9) and proinflammatory cytokines (TNF- α, IL-1 β, IL-6 and IL-8) and whether transfusion of shed blood increases the concentrations of inflammatory mediators in the circulation. 2. Twenty consecutive patients undergoing total hip replacement surgery under spinal anaesthesia were studied. The patients were transfused with whole blood collected postoperatively. 3. The median volume shed blood returned to the patients was 350 ml (25–75% range=300–450). Before transfusion of shed blood was filtered using a 40 μm filter (Solcotrans®). Samples for complement and cytokine determinations were drawn from the collected blood. 4. Venous blood samples were drawn 1 min before transfusion, 1 and 60 min after completed transfusion. High concentrations of C3a, SC5b-9, TNF- α, IL-1 β, IL-6 and IL-8 were found in shed blood. The concentrations were higher than the circulating levels ( P<0.05). The filtration procedure did not significantly reduce the concentrations. 5. Transfusion of the shed blood did not significantly alter the circulating concentrations of C3a, SC5b-9, TNF- α, IL-1 β, and IL-8. The plasma concentrations of IL-6 were increased both 1 and 60 min after completed transfusion compared to before ( P<0.05). 6. This study shows that whole blood collected from a surgical wound contains large concentrations of complement split products and proinflammatory cytokines. Transfusion of shed blood leads to elevated plasma levels of IL-6.