Formation of cartilage in aortic valves of Syrian hamsters

Abstract

The formation of cartilage in aortic valves of Syrian hamsters was studied using histological, histochemical and immunohistochemical techniques. The sample consisted of 281 specimens aged 0-363 days, all of which had a normal (tricuspid) aortic valve. The first sign of valvular chondrogenesis is the presence of small groups of cells embedded in a type II collagen-positive matrix. These groups of cells, which can appear as early as one day after birth, increase in size and differentiate into hyaline cartilage or fibrocartilage. From the fourth day of life, all hamsters examined displayed cartilaginous foci in the aortic valve. They were located along the fibrous attachments of the valve leaflets to their respective sinuses, including the valve commissures. A considerable proportion (76%) of cartilages formed within the first 40 days of life, that is during the period of time in which the histogenesis of the valve takes place. The present observations are consistent with the assumption that in mammals, the precursors of the aortic valve chondrocytes are neural crest-derived cells. Results of a statistical analysis substantiate that the incidence is significantly higher in (1) the territory that comprises the collagenous condensation of the ventral commissure and the ventro-lateral and proximal fibrous attachments of the right leaflet to its sinus, and (2) the proximal fibrous attachment of dorsal leaflet to its sinus. These findings together with data in the literature concerning the distribution of stress in each leaflet-sinus assembly of the valve during the cardiac cycle, suggest that mechanical action might play an inductive role in the formation of the cartilaginous tissue in the aortic valve of mammals. In addition, they point to the possibility that locally intense mechanical stimulation is responsible for the differentiation of the anticipated cartilaginous tissue into hyaline cartilage.