Abstract
Bilayer or black lipid membranes (BLM) have been produced from a wide variety of materials and their physical properties extensively investigated ( Haydon and Taylor, 1968; Henn and Thompson, 1968; Howard and Burton, 1968m; Lauger and Richter, 1968; Ohki and Goldup, 1968). A review of the work on BLM has recently appeared ( Tien and Diana, 1968). In order to study membrane-associated phenomena relevant to photosynthesis and related processes, we decided to form BLM from photosynthetic pigments and lipids which hitherto have not been used alone in the BLM formation. This communication describes detailed procedures for the preparation of lipid solutions from spinach chloroplasts for BLM formation. In addition, a summary of the measured physical properties of these new BLM is reported.
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