Formation of active oxygen species by dithranol, III. Dithranol, active oxygen species and lipid peroxidation in vivo.

Abstract

AbstractAt pH 7.8 the dithranol anion 1a is oxidized in the dark to yield the bianthrone 3 besides dithranol brown, whilst O2 is reduced to O2·− and H2O2. O2·− was detected by the reduction of nitroblue‐tetrazolium (NBT, 4). The formation of the formazan 5 was inhibited by superoxide dismutase (SOD). Exclusion of O2 and addition of SOD reveal a direct electron transfer from 1a to 4 and to cytochrome c which was not affected in the presence of SOD. — In vivo experiments indicate a low rate of lipid peroxidation which was determined on the basis of ethane exhalation.