Abstract
Background. The application of convalescent plasma (CP) is currently seen as a feasible therapeutic approach in the treatment of COVID-19.Aim. To analyze the experience of recruiting a donor cohort from COVID-19 convalescents for banking of CP as part of a pilot project at the Moscow Healthcare Department.Materials and methods. A retrospective research included 493 COVID-19 convalescents as potential CP donors, all examined at the Sklifosovsky Research Institute for Emergency Medicine. CP was banked using the plasmapheresis method. Only those donors with a documented medical history of COVID-19, which was confi rmed by polymerase chain reaction of SARS-CoV-2 RNA in pharyngeal swabs, and no sooner than 14 days after complete recovery were eligible for donation. Viral neutralizing activity (VNA) was chosen as the key characteristic of the immunological viability of CP. All the donors having VNA titers were characterized in terms of gender, age, time interval since the disease onset, regression of clinical symptoms and clinical features of the COVID 19 course.Results. Effective (1:160 or more) and acceptable (1:80) VNA titers were found in 21.1 % and 24.75 % of donors, respectively. Signifi cant predictors for a donor having a high VNA titer included: male sex, age over 36 years and verifi ed viral pneumonia. The absence of a signifi cant body temperature response (38.5 °С) can be considered as a negative marker of a potential donor.
Highlights
The application of convalescent plasma (CP) is currently seen as a feasible therapeutic approach in the treatment of COVID-19
Distribution of donors depending on the Viral neutralizing activity (VNA) titer in blood plasma
Klein S., Pekosz A., Park H.S. et al Sex, age, and hospitalization drive antibody responses in a COVID-19 convalescent plasma donor population
Summary
В рамках пилотного проекта Департамента здравоохранения города Москвы в ретроспективное исследование включено 493 реконвалесцента COVID-19, прошедших обследование в ГБУЗ «НИИ скорой помощи им. Период от начала заболевания и время, прошедшее с момента выздоровления, были одними из основных критериев отбора потенциального донора-реконвалесцента для проведения плазмафереза. При однофакторном анализе взаимосвязи интервала от начала и окончания заболевания с величиной титра ВНА были обнаружены: отсутствие зависимости от периода после выздоровления до плазмафереза (коэффициент корреляции r = 0,058675, p = 0,2) и слабая, но достоверная зависимость титра ВНА в пробе крови донора перед донацией от продолжительности периода, который прошел с момента появления первых симптомов до плазмафереза (r = 0,13, p = 0,0042, 95 % доверительный интервал (ДИ) 0,041597–0,218293). Для анализа зависимости содержания ВНА у донора от периода, прошедшего с дебюта новой коронавирусной инфекции до донации плазмы, все реконвалесценты были разделены на 5 групп в соответствии с количеством дней, прошедших от дебюта заболевания до плазмафереза: 1-я группа — до 20 дней, 24.75%. Distribution of donors depending on the VNA titer in blood plasma
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