Abstract
Doubly labeled phytic acid of high specific activity was prepared by incubating whole wheat seeds with [ 32P]phosphoric acid and [ 3H]myoinositol for 48 h and purifying by anion-exchange chromatography on AG 1-×8 resin. Both degradation and synthesis of phytic acid were inhibited by KF to a similar extent, yet the catabolic and anabolic pathways involved distinctly different enzyme systems, as no [ 3H, 32P]myoinositol tetra- or pentaphosphate could be detected.
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