Abstract
To raise the yields for the production of 14C-labelled zearalenone in Fusarium cultures the influence of growth conditions and known effectors or precursors of toxin biosynthesis was studied. Benzoic acid and 2,4-dihydroxybenzoic acid used as precursors decreased toxin formation; in the presence of different pesticides such as 2,4-dichlorophenoxyacetic acid, however, toxin production increased up to 140%. The known pathway of zearalenone biosynthesis could be confirmed from the relative extents of 13C-incorporation into the zearalenone molecule by incubating Fusarium graminearum DSM 4529 with d-(+)-[1-13C]glucose as carbon source. When grown in the presence of d-[U-14C]glucose or [2-14C]malonic acid the strain produced [14C]zearalenone with specific activities of 0.07 and 0.09 μCi/mg, the 14C-incorporation rates being 0.34% and 0.48%, respectively.
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