Formation and Microscopic Appearance of Fireworks-like Objects Created from Synthetic DNA (E. coli) Crown Cells with Adenosine-DNA (E. coli)

Abstract

Techniques for producing artificial cells (DNA crown cells), in which the outer membrane surface is covered with DNA were established in 2012. Such cells, hereafter referred to as synthetic DNA crown cells, can be readily synthesized using sphingosine (Sph)-DNA-adenosine mixtures and can proliferate within egg white or in vitro. However, how synthetic DNA crown cells proliferate within egg white or grow in vitro has not yet been clarified in detail. Synthetic DNA (E. coli) crown cells formed assemblies, which were reconstructed either spontaneously or in the presence of salts. The formation of new compounds, such as adenosine-DNA compounds, may occur within the reconstructed assembly. In this study, adenosine-DNA (E. coli) compounds were first prepared then the possible effect on adenosine-DNA (E. coli) by cell formation was investigated. The present experiments showed that fireworks-like objects were formed from synthetic DNA (E. coli) crown cells together with adenosine-DNA (E. coli) compounds. The findings suggested that adenosine-DNA played a role in the new of cell formation mechanism and participated in cell generation.