Abstract
The formation and degradation of Δ9-1,18-octadecenedioic acid from oleic acid by mutant S76 of Candida tropicalis was studied. It was found that the mutant could convert oleic acid to Δ9-1,18-octadecenedioic acid by ω-oxidation of the terminal methyl group. The substance was identified by various analytical methods and could be degraded to unsaturated dioic acids of shorter chain length with even numbers of carbon atoms from 6 to 16, and saturated dioic acids with 6, 8 and 10 carbon atoms. Neither unsaturated nor saturated dioic acids with odd numbers of carbon atoms were found in the media. From these results some metabolic pathways of the degradation of this unsaturated dioic acid are proposed. Some mass spectra of unsaturated dioic acids are presented.
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